|Koordinator||Karolinska Institutet - Department of Medical Biochemistry and Biophysics|
|Bidrag från Vinnova||1 617 864 kronor|
|Projektets löptid||juli 2017 - juni 2019|
Syfte och mål
The research I propose will aim to add my contribution to providing a transciptome map of epithelial cells in the mammal body’s inner and outer protecting layer: intestine and skin. The goal of these investigations is to determine the differences in the metabolism of stem cells (healthy dividing cells) and cancer cells (multiplication due to pathogenic metabolism). The motivation for the project is that it is a widely accepted theory that malignant cell growth is caused by differentiated cells reverting back to stem cells, hence gaining unlimited mitotic capabilities.
Förväntade effekter och resultat
Here I propose novel methods for automated cell detection and detection of nanoscale barcodes attached to the same cells. Cells are visualized by optical microscopy and labelled using opto-chemical barcodes. Chemical identification is made possible by the diversity of the DNA oligomers that the barcodes consist of and optical identification by fluorescence. Having the functional goal of finding connections between altered transcriptomics and morphological changes, the experimental targets were chosen to be functional units of intestinal crypts.
Planerat upplägg och genomförande
I aim to solve the problem of aligning images at multiple scales, and on the computational challenge of identifying DNA barcodes and determining which barcodes belong to which cells. Regarding my contribution, I have three specific aims: assignment of barcodes to cells, imaging at different scales, and establishing the correct method for surface functionalization.