Nanoteknik möter cellbiologi: Betydelsen av cellmembranets form vid hormonutsöndring

Diarienummer 2015-01541
Koordinator Uppsala universitet - Institutionen för medicinsk cellbiologi
Bidrag från Vinnova 1 048 803 kronor
Projektets löptid juli 2015 - september 2016
Status Avslutat

Syfte och mål

Throughout the process of insulin secretion, the shape of the membranes change dramatically, but no methods exist for quantitatively studying membrane shape and protein interactions in live cells. To develop new methodology in this interdisciplinary project we had two main objectives: 1) To develop substrates for cellular growth that introduce membrane curvature into cells and 2) To assess how docking of insulin containing vesicles and the recruitment of fusion proteins are affected by the newly introduced regions of membrane curvature.

Resultat och förväntade effekter

The results of this work led to the design of novel methods for the nanofabrication of glass substrates. The electron beam lithography process of nanofabrication designed here yields substrates that are useful for imaging cell membranes with total internal reflection fluorescence microscopy, a common tool of cell biology. Finally, using these substrates, insulin secreting cells were cultured with fluorescently labeled insulin granules and SNARE proteins to address the role of membrane curvature in insulin secretion.

Upplägg och genomförande

The experimental design involved patterning substrates for cellular growth. Once insulin secreting cells were grown on substrates, quantitative microscopy methods were used to determine if the cell conforms around the substrate. Ultimately, protein organization will be quantified. Funding from Vinnova also affected the mobility of the researcher. The researcher connected with Swedish scientists at Uppsala Univeritet, Stockholm Universitet and Lund Universitet with the purpose of establishing future collaborations in grant applications and student exchange.

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