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Targeted NGS for high-throughput veternary parasitology diagnostics

Reference number
Coordinator Vidilab AB
Funding from Vinnova SEK 329 000
Project duration December 2017 - April 2019
Status Completed

Purpose and goal

The aim was to develop a more sensitive, scalable and automatable method for detection of pathogen parasites in horses. While the tested method did not show promise during the project a new method which also offers all of the benefits mentioned above did show promise.

Expected effects and result

The method tested showed problems with detection of a large fraction of the positive samples. The cause of this was identified during the project as well as a possible solution. However this would include a price too high for most customers. Instead one of the comparison methods are being tested as this had a smaller fraction of false negatives.

Planned approach and implementation

Initially the parameters and conditions for the method was optimised using a small set of example samples. This method was then compared with two similar methods representing different stages of the investigated method. The comparison was made on 280 samples from horses from all over Sweden to represent the geographic variations as well as variations in infection burden.

The project description has been provided by the project members themselves and the text has not been looked at by our editors.

Last updated 18 December 2018

Reference number 2017-04328

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