Formulation of recombinant spider silk into matrices for cell culture

Purpose and goal

Recombinant spider silk protein can be used for production of matrices for cell cultivation and tissue engineering. The purpose of this project was to study the effect on matrix formation by varying two key process parameters. This knowledge forms the basis for the design of an automated manufacturing process and selection of appropriate analytical methods for routine control of matrix quality. We have also initiated discussions with suppliers of pipetting robots. However, we have not yet decided on patenting.

Results and expected effects

Two parameters can be varied during fabrication of spider silk protein matrices. They effect matrix properties and must accounted for during design of the manufacturing method. The following properties were studied during this project: time for film formation, thickness, viscoelasticity, hydrofobicity, secondary structure and surface morphology. These properties affect matrix strength and bicompatibility. We consider implementing hydrophobicity and surface morphology as controls to assure product consistency.

Approach and implementation

Protein solutions were produced by Spiber Technologies AB according to current protocols. Matrices were fabricated under defined conditions with variations in one parameter at a time. Analyses were performed by SP Technical research institute of Sweden: Chemistry, material and surfaces (formerly Institute of surface chemistry). Some of the methods used were Raman spectroscopy, FTIR_ATR and scanning electron microscopy. Results were evaluated and discussed continuously to guide new experiments. All results were summarised in a report.