Proteomics and aging biomarkers at Uppsala
|Coordinator||Stockholms universitet - Institutionen för biokemi och biofysik|
|Funding from Vinnova||SEK 1 880 000|
|Project duration||December 2008 - April 2012|
Purpose and goal
Proteomics and aging biomarkers at UppsalaThis programme will allow me to develop a long-term collaboration between my research group and one of the world-class groups in the field of clinical proteomics and neuroscience leaded by Prof. Jonas Bergquist at the department of Analytical Chemistry, Uppsala University. During my stay at the guest environment, I will explore shotgun proteomics techniques to apply to the project proposed here:Proteomics and aging biomarkers. Moreover, back to my research group, we will have the opportunity to extend these strategies to several projects dealing with environmental proteomics and novel biomarkers for pollution assessment, an area where we are one of the leaders of the field.
Expected results and effects
The specific aim of this project is to identify candidate aging-biomarkers from animal models that could also facilitate understanding of age-related diseases. The specific aims are: 1.Search for aging biomarkers through the use of shutgun proteomics and isobaric tags for quantification. 2.Analyse gender specific response to aging. 3.Study the effect of long-life treatment with antioxidants in the response to aging.
Planned approach and implementation
Methodology: 1. Shotgun proteomic methods involving isobaric tagging of peptides that enable simultaneous identification and quantification of peptides using tandem MS such as iTRAQ. 2. Peptide profiling methods are a novel, automated technology platform for the simultaneous measurement of serum peptides that generates highly reproducible patterns.