High throughput test and procedures for ultra sensitive and accurate chimerism analysis in transplant patients
| Reference number | |
| Coordinator | Devyser AB |
| Funding from Vinnova | SEK 2 000 000 |
| Project duration | February 2018 - January 2019 |
| Status | Completed |
| Venture | Innovationsprojekt i företag 2017 |
Purpose and goal
The purpose of the project was to develop an NGS-based method for analyzing chimerism after bone marrow transplantation. We have now, at the end of the project, managed to develop such a method for RUO (Research Use Only) use but have also started work on validating the method according to IVDR-CE.
Expected results and effects
We have managed to develop a sensitive method that can detect residual patient cells after a bone marrow transplant down to a level of 0.1%. In addition, the method has good precision both at low and high levels. It would thus be able to replace the commercial methods used today. The method has been tested on labs in Sweden and abroad with great success.
Planned approach and implementation
We have bioinformatically searched for possible genetic markers to be able to distinguish between different individuals. After testing a number of markers, we have found 24 markers that are sufficiently specific and sensitive to be used in the test. In connection with the project, we have also developed a software for calculating and visualizing results.