EPIGRAPH

Purpose and goal

EPIGRAPH proposed graphene-based biomolecular sensors, organic electronic ion pumps (OEIP) neurotransmitter delivery, and electrophysiological electrodes integrated into a device platform/toolkit for the prediction and control of epileptic seizures. The main objectives were to: (i) develop graphene-based sensors using state-of-the-art laser processing; (ii) control brain activity via graphene-based OEIPs; and (iii) integrate biochemical sensors, OEIPs, and electrophysiological sensors into a single device platform. Goal iii is still under development.

Expected results and effects

A variety of new bioelectronics were developed. One such, the microfluidic-ion pump, represents hybridization of “iontronics” with microfluidics, combining the best of both worlds. Another such technology was glass-capillary based ion pumps. Finally, we developed pulled pipette-based ion pumps, based on glass micropipettes widely used in neuronal electrophysiology research, with tip dimensions below single-cell scale. These devices are suitable for brain slice and single-cell experiments, and open a new paradigm of precision non-flow delivery for cell signalling studies.

Planned approach and implementation

This FLAG-ERA project spanned partners in Sweden (LiU, RISE, where LiU was international coordinator), France (AMU), and Greece (FORTH). LiU/Daniel Simon coordinated both the Swedish activities and the project in general. We encountered several hindrances due to the pandemic which contributed to delays in reaching objective iii. However, we were able to maintain coordinated international effort, resulting in several publications and follow-up proposals.