Quantitative insulin inside individual vesicles with nano-tip electrodes: Is insulin exocytosis all or partial?

Important results from the project

** Denna text är maskinöversatt ** Diabetes is one of the most common diseases in the EU. In this project, as the main goal, we have tried to determine whether beta cell vesicles release some of their insulin content or all during exocytosis or not. We have also tried to find a new way to study the level of insulin secretion during rapid exocytosis for individual beta cells. To achieve these objective, we have used a new analytical technique and archived the new results. Finally, we believe that, in addition to training the applicant in various aspects, we can achieve most of the determined goals.

Expected long term effects

** Denna text är maskinöversatt ** The new tools for cellular analysis in diabetes research have been developed. As results, we have found that the "partial release" theory is the approach for exocytosis in beta cells, and the level of vesicle opening in cells with diabetes is different from healthy cells. Both findings have a direct impact on the amount of insulin release and diabetes, and may lead to a new insight into insulin release and development for a diabetes (T2) treatment in the future. Also, the discoveries have been transferred to the Sahlgrenska Academy for further experiments.

Approach and implementation

** Denna text är maskinöversatt ** The project consist of four work plans (1-4) for two years. In summary, (1) Applicant learns new abilities / skills relevant to the projects. (2) Nanosensors were applied to quantitative analyzes and fraction of secretion from individual beta cells was determined. (3) The dynamics of pore openings and chemical regulation of exocytosis were studied. (4) The results were presented at a conference and reported to research and review articles (Open access).