Systematic technology development of chemicals for freezing live blood

Purpose and goal

The project aims to develop an optimised cryogenic preservation method for whole blood that maintains the natural composition and activation state of immune cells. By using Differential Scanning Calorimetry (DSC) to analyse and refine cryoprotective formulations, the goal is to overcome the limitations of current PBMC-based freezing protocols and enable reproducible, high-quality whole-blood biobanking.

Expected effects and result

The project is expected to deliver a validated cryopreservation protocol that minimises cell damage from ice crystallisation and cryoprotectant toxicity. This innovation will allow large-scale, multi-centre studies using cryopreserved whole blood with preserved cellular function, improving data quality, comparability, and access to clinical samples in immunological research.

Planned approach and implementation

The project will use DSC as a central analytical tool to evaluate thermal properties of cryoprotectants and freezing behaviour. Iterative testing and optimisation will guide the design of novel buffer compositions. Collaboration between scientific and technical partners will ensure translation from experimental validation to robust protocols suitable for clinical research environments.