Ultrasensitive detection of fusion oncogenes in liquid biopsies of pediatric cancer

Important results from the project

** Denna text är maskinöversatt ** The project aimed to combine the two-tailed PCR and SimSen sequencing techniques to develop sensitive assays for the detection of cancer-related fusion oncogenes. These assays would be evaluated in terms of specificity, sensitivity and possibility of multiplex. The produced assays should also be prepared for commercialization.

Expected long term effects

** Denna text är maskinöversatt ** Assays for the three most relevant fusion oncogenes in childhood cancer have been developed and validated based on a combination of the two-tailed PCR and SimSen sequencing techniques. This has resulted in two patent applications. A company for the commercialization of applications has been started and discussions are ongoing with external partners about collaborations around the technologies.

Approach and implementation

** Denna text är maskinöversatt ** The project has been carried out in collaboration between TATAA Biocenter, Göteborgs University and Sahlgrenska hospital. Technology exchange and assay development have taken place in close collaboration between TATAA Biocenter and Göteborgs University, as well as Sahlgrenska Sjukhuset with relevant clinical information for the assay design. Sahlgrenska Sjukhuset has collected material for evaluation of the designed assays. All partners have also been involved in the set-up for commercialisation.