Platform for the personalization of xenogeneic and 3D printed tissue scaffolds

Important results from the project

The project aimed to develop personalized tissues using xenogeneic material (from other species) or 3D-printing instead of human donor tissue. We developed a method enabling the use of material from other species (cow, pig, and sheep) and demonstrated that these can be transplanted without complications. We also produced tubular structures using 3D printing. The model organs have been blood vessels and urethras, but the platform we created is generic and can be applied to many other organs and tissues.

Expected long term effects

We developed a method where blood vessels from different species (sheep, pig, cow) were decellularized and personalized with blood from a recipient sheep that then got the blood vessel transplanted. The studies showed that the transplants were safe and functional without side effects, and these results will now support an application for clinical trials using xenogeneic material. We also created 3D-printed tubular structures for urethra and blood vessels, which are ready for testing in animal models after the project’s completion.

Approach and implementation

The project followed the plan: In WP1, we developed bioinks for 3D-printed tubular structures, printing urethras and blood vessels. In WP2, we established a method for personalizing xenogeneic material from sheep, cow, and pig. WP3 validated the safety and functionality of xenogeneic grafts in an in vivo sheep model for artery transplantation. We also secured ethical approval to transplant 3D-printed structures post-project. WP4 handled regulatory management, and data collection. The project has been disseminated at conferences, and three manuscripts are in preparation.