EcCustomIB for customizing E. coli for the production of recombinant proteins in inclusion bodies

Important results from the project

The EcCustomIB platform for the customisation of E. coli for the production of recombinant proteins in inclusion bodies was developed and validated. Importantly, each recombinant protein tested indeed required its own customised strain for its optimal production in inclusion bodies. In addition, we have initiated generating our own strains to which no licensing strings from other parties are attached. Having our own strains will significantly facilitate commercialising EcCustomIB.

Expected long term effects

The technology to customise E. coli for the production of recombinant proteins in inclusion bodies has now been developed/validated. It has become clear to us that in order to start a company that commercialises this technology, it will also need its own well characterised/passaged E. coli strains that can be used without the involvement of any other parties for the production of any recombinant protein. We have now obtained strains that can be used to generate such strains.

Approach and implementation

The EcCustomIB platform was developed/validated. During the project we made contact with potentially interested parties. This made it clear that there is definitely an interest in EcCustomIB. However, it also became clear that having our own E. coli strains would be extremely helpful to commercialise the EcCustomIB technology. Efforts to generate our own strains are in full swing.