Probing the dynamic processes in freeze-drying in-situ
| Reference number | |
| Coordinator | RISE Research Institutes of Sweden AB - RISE Bioscience and Materials / Chemistry, Materials and Surfaces Unit |
| Funding from Vinnova | SEK 98 190 |
| Project duration | June 2019 - October 2019 |
| Status | Completed |
| Venture | Research infrastructure - utilisation and collaboration |
| Call | Industrial pilot projects for utilisation of neutron- and photon based techniques at large scale infrastructures - spring 2019 |
| End-of-project report | 2019-02562_BioGaia.pdf (pdf, 387 kB) |
Important results from the project
We have made a literature study and identified neutron techniques to study dynamic processes during freeze-drying and rehydration in situ. We want to watch water through the process, and look at the spatial distribution of the probiotic lactobacilli in the freeze concentrated matrix, and how this distribution can be altered by the different drying protectants and over time. BioGaia´s goal for is an optimized freeze drying process through increased understanding of the governing factors
Expected long term effects
Our research proposal for experimental measurement of the freeze drying process, along with relevant sample environment development aimes at improving the processes that BioGaia utilizes. The pre-study has also generated collaborative ideas for the expertise at Uppsala University and RISE to present for other industry partners for them to engage in scientific studies related to freeze-drying of biological materials.
Approach and implementation
We have identified appropriate neutron-based techniques (tomography, diffraction and USANS) and experimental questions to be answered as well as design for sample environment set-ups to solve problems related to freeze drying. We have selected a suitable research facility and collaborative partner and have written and submitted a beam time application. We have also identified that in the long run it may be suitable to extend the study using synchrotron techniques.