Microparticle based assay for the diagnosis of bleeding disorder

Purpose and goal

The goal of the project is to develop a modern test to diagnose von Willebrand (vW) disease. The tests is based on the use of microparticles and can be performed by automated coagulation instruments. We have developed a method in which proteins can effectively be bound and oriented to a surface under controlled conditions. We have used this method to orient and attach a receptor for von Willebrand factor onto microparticles and have shown that these receptor-linked particles can be used in a test to measure vWF activity.

Expected results and effects

We have developed a method in which proteins can effectively be bound and oriented to a surface under controlled conditions. We have verified that the method works and provides a good basis for developing a test for von Willebrand factor in patient plasma. We are now well on the way to a large scale production of reagents for this test and bring the product to market. With the finished product Nordic Biomarker becomes one of only three manufacturers of this test in the world. It will give us a strong position and contribute to revenue growth in the coming years.

Planned approach and implementation

The project has largely followed the project plan. When we chose to design and order the different receptor variants in series instead of in parallel as we originally thought the project took more time than scheduled. We decided that it was better to exploit the experiences of the first types to make better design on the latter. This proved to be successful and the latest receptor was shown to work best and be the one we chose to move forward with. Everything has worked technically well in the project. However, a lesson learned is that it´s easy to become too time optimistic.