Biocatalysts for stereoselective synthesis/kinetic resolution of phosphodiesters

Purpose and goal

The aim of this study was to investigate the feasibility of a biocatalyst for synthesis of enantiomerically pure phosphate esters, which are valuable pharmaceutical compounds (e.g. antiviral drugs). Two active enzymes for the desired reaction were identified. We have also shown that our in-house developed technology for binding enzymes to a carrier (for enzyme reusal) is functional and useable in a chemical flow system, thereby appliable for large-scale processes. Further, an investigation of the market is ongoing, where the initial results show a significant customer base.

Results and expected effects

A new set of biocatalysts for phosphate ester-containing reactions is under development and initial proof-of-concept reactions have been performed. An inventory of the market has been started. An propriatory technique for enzyme immobilization has been applied and tested for scalalability in synthetic chemistry. This will increase the market interest not only for the present catalyst system, but also for the products of the company in general.

Approach and implementation

1. Theroretical studies for identification of suitable model reactions Model reactions established 2. Experimental verification of the catalyst Desired activity was found in two enzymes, enzyme engineering is to be performed. 3. Assesment of suitability and potential for large-scale applications Experiments showed that the technology is feasible for large-scale processes. 4. Investigation of the market A customer base was found, a business model is under development and continued market evaluation is needed. 5. Patenting and preparation for commercialisation Ongoing