AFISmon: Development of an autonomous multisampler system for the monitoring of biogeochemical processes
| Reference number | |
| Coordinator | KUNGLIGA TEKNISKA HÖGSKOLAN - Science for Life Laboratory |
| Funding from Vinnova | SEK 438 634 |
| Project duration | January 2014 - March 2017 |
| Status | Completed |
Purpose and goal
Microorganisms are the driving catalysts for biogeochemical cycles in the ocean. With metatranscriptomics - analysis of mRNA in microbial communities - you can read which biogeochemical processes are active in a seawater sample. However, the transcripts break down quickly, which complicates the analysis. The project aims to develop an automatic water sampler that chemically conserves mRNA in the sample when taken. The sampler can sit on e.g. a buoy and conserves the sample for a number of days for later retrieval. Protocols have also been optimised for optimal sample preservation.
Expected results and effects
We have successfully developed a well-functioning prototype for autonomous water sampling that conserves mRNA in the sample at the sampling occasion.
Planned approach and implementation
Protocols for optimal conservation of RNA have been evaluated on artificial as well as on real microbial communities (seawater samples) by analyzing the quality and quantity of mRNA after conservation and by comparing the metatranscriptomic profile before and after mRNA preservation.