Magnetic beads for purification of monoclonal antibodies and cells
| Reference number | |
| Coordinator | Lab-on-a-bead AB |
| Funding from Vinnova | SEK 400 000 |
| Project duration | May 2020 - December 2020 |
| Status | Completed |
| Venture | Competence centre |
Important results from the project
Initially, the magnetic ion exchange particles developed in SMF 2018-03120 would be evaluated for the purification of Erythropoietin (EPO). In parallel, the analyzes of Host Cell Protein (HCP) in purified product previously planned would now be completed and results now communicated via publication. Finally, magnetic particles would be developed for the purification of human stem cells.
Expected long term effects
The project´s goals have generally been achieved. The magnetic particles have now been evaluated at KTH. However, the performance has not been satisfactory. That is why we have chosen to change ligand. The HCP levels in purified antibody have been satisfactorily analyzed and this part has now been summarized as a manuscript which undergoes a Review process in the journal Biotechnology and Bioengineering 201096. A patent application HIGH CELL DENSITY (PCT / EP2020 / 077861) has also been filed. Magnetic beads for purifying human stem cells are under development with AstraZeneca.
Approach and implementation
The project has been a collaborative project between MAGic BioProcessing (Lab-on-a-Bead), KTH and Astra Zeneca, where MBP has developed magnetic beads for purification of EPO via ion exchange and magnetic beads for purification of human stem cells. At KTH, the planned HCP analyzes have been carried out and the purification of EPO with ion exchange beads has been evaluated. Together, the manuscript and the patent application have been summarized. AZ has provided MBP with cell-specific antibodies for coupling to their magnetic beads.