A Novel multijunction capillary isoelectric focusing fractionator for proteomics
|Coordinator||Karolinska Institutet - Department of Medical Biochemistry and Biophysics|
|Funding from Vinnova||SEK 1 756 281|
|Project duration||September 2015 - September 2017|
Purpose and goal
The main goal was to create a new analytical separation device for the proteomics laboratory, the pI-Trap. This has been done, and Biomotif has already delivered 5 instruments to different clients, including one to KI, where the instrument underwent thorough testing and validation.
Expected results and effects
The project has been successfully implemented. A novel device for fractionation of peptides and proteins in solution by isoelectric point, the pI-Trap, has been designed and built. The instrument has found small but growing market, with several commercial instruments delivered to customers. The response of the customers is overwhelmingly positive. On the scientific side, the pI-Trap has been used in a number of applications.
Planned approach and implementation
Especially attractive was the use of the pI-Trap for TMT-labeled peptides, as such peptides represent the majority of samples in modern shotgun proteomics. The experiment showed that the pI-Trap can separate TMT-labeled peptides as well as unlabelled peptides. Capitalising on this success, two novel versions of the pI-Trap are foreseen - the fully automated preparative version with an autosampler, to be designed, built and marketed together with MS Vision (Netherlands), and an analytical version optimised for analysis of blood proteins in the clinical area of Alzheimer´s disease.