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A Novel multijunction capillary isoelectric focusing fractionator for proteomics

Reference number
Coordinator Karolinska Institutet - Department of Medical Biochemistry and Biophysics
Funding from Vinnova SEK 1 756 281
Project duration September 2015 - September 2017
Status Completed
Venture Eurostars

Purpose and goal

The main goal was to create a new analytical separation device for the proteomics laboratory, the pI-Trap. This has been done, and Biomotif has already delivered 5 instruments to different clients, including one to KI, where the instrument underwent thorough testing and validation.

Expected results and effects

The project has been successfully implemented. A novel device for fractionation of peptides and proteins in solution by isoelectric point, the pI-Trap, has been designed and built. The instrument has found small but growing market, with several commercial instruments delivered to customers. The response of the customers is overwhelmingly positive. On the scientific side, the pI-Trap has been used in a number of applications.

Planned approach and implementation

Especially attractive was the use of the pI-Trap for TMT-labeled peptides, as such peptides represent the majority of samples in modern shotgun proteomics. The experiment showed that the pI-Trap can separate TMT-labeled peptides as well as unlabelled peptides. Capitalising on this success, two novel versions of the pI-Trap are foreseen - the fully automated preparative version with an autosampler, to be designed, built and marketed together with MS Vision (Netherlands), and an analytical version optimised for analysis of blood proteins in the clinical area of Alzheimer´s disease.

External links

The project description has been provided by the project members themselves and the text has not been looked at by our editors.

Last updated 5 September 2019

Reference number 2015-04208

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